4peaks
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Description:
Visualise and edit DNA sequence trace files
Type: Cask  |  Latest Version: 1.8@0  |  Tracked Since: Dec 17, 2025
Links: Homepage  |  formulae.brew.sh
Category: Developer tools
Tags: bioinformatics dna-sequencing sanger molecular-biology chromatogram
Install: brew install --cask 4peaks
About:
4peaks is a specialized macOS application designed for molecular biologists to visualize and analyze DNA sequence trace files generated by Sanger sequencing. It provides intuitive tools for viewing chromatograms, editing sequence data, and assigning base calls to resolve ambiguities. The software is essential for quality control and manual editing of sequencing results before further analysis.
Key Features:
  • Chromatogram visualization with color-coded base calls
  • Manual editing of sequence data and base assignments
  • Support for multiple file formats including AB1 and SCF
  • Sequence alignment and comparison tools
  • Quality value analysis and display
Use Cases:
  • Quality control of Sanger sequencing results
  • Manual editing and correction of base call errors
  • Comparing sequenced fragments to reference sequences
Alternatives:
  • SnapGene – Commercial tool with broader molecular biology features but higher cost
  • UGENE – Free open-source suite with trace viewing but less specialized interface
  • FinchTV – Free alternative with similar trace viewing capabilities
Version History
Detected Version Rev Change Commit
Aug 5, 2025 12:47pm 1.8 0 VERSION_BUMP db6e9565
Jul 9, 2024 10:13am 1.8 0 VERSION_BUMP c7da1bd4