primer3 ☆
« Back to VersTracker
Description:
Program for designing PCR primers
Type: Formula  |  Tracked Since: Dec 28, 2025
Links: Homepage  |  formulae.brew.sh
Category: Developer tools
Tags: bioinformatics genomics pcr primers cli
Install: brew install primer3
About:
Primer3 is a command-line tool for designing and analyzing PCR primers. It automates the selection of optimal primer pairs based on parameters like melting temperature, GC content, and specificity. This enables researchers to efficiently generate high-quality primers for DNA amplification and genotyping.
Key Features:
  • Automated primer design and analysis
  • Customizable parameters for specificity and product size
  • Handles common tasks like tiling and SNP detection
  • Robust command-line interface for scripting
Use Cases:
  • Designing primers for DNA sequencing or cloning
  • Creating genotyping assays for genetic markers
  • Optimizing existing primer sets for improved performance
Alternatives:
  • Primer3Plus – Web-based graphical interface for Primer3 that offers enhanced visualization and workflow.
  • NCBI Primer-BLAST – Integrates primer design with BLAST to ensure specificity against reference genomes.
Version History
Detected Version Rev Change Commit
Sep 15, 2025 7:10am 0 VERSION_BUMP 5384aecd
Sep 12, 2024 7:39pm 0 VERSION_BUMP ec2f4055
« Back to VersTracker  |  Browse Packages  |  About